While it is a good idea to assume someone owns a domain just because they have an email there is on the right track, it is not always correct. Personally, I use Gmail as one of my primary email accounts. If you disallow me signing up using that account it's your loss, not mine.
My suggestion which works for a corporation I program for, only disallow from specific outlets, like anything from .ru, ip address neighborhoods, etc. Banning a huge provider like that is just shooting yourself in the foot.
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