Your thread title suggests you were asking if you can pull a PCI card out of it's slot on the mother board while it's running.
Dont do that.
If your cdrom and/or harddrive controller is that flaky, then maybe your problem is related to the seating of the PCI card. But turn the shit off and wait a minute before you yank it out. Blow the dust out, and reseat it, lol.
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